Impact of the LPS from Cyanobacteria <i>Brasilonema sp.</i>on Lung Inflammation and Glucocorticoid Expression

Abstract Inhaled glucocorticoids (GCs) are the first-line treatment for asthma. However, patients with asthma characterized by recruitment of neutrophils show poor sensitivity to GC therapy. A potential etiology this GC-resistant is aerosolized cyanobacterial lipopolysaccharide (LPS) from blooms. Previously, we demonstrated that inhalation LPS Geitlerinema sp. cyanobacteria induces neutrophilic lung infiltration in mice. Our current data shows another cyanobacteria, Brasilonema sp., also inflammation after inhalation. Flow cytometric demonstrate neutrophil and ELISA identified increases pro-inflammatory cytokine levels bronchoalveolar fluid exposure. Furthermore, IHC mice exposed an increased level epithelial GCRβ, a glucocorticoid receptor (GR) analog inhibits activity. Conversely, expression GCRα, GR isoform responsible sensitivity, remains unchanged. These help elucidate mechanisms LPS-induced lung-inflammation resistance, while highlighting importance toxin promoting human disease near This work supported Kenneth A. Suarez Fellowship Intramural Funds at Midwestern University.